Multicolor flow cytometry guide

Quickly set up your flow cytometry experiment with our comprehensive guide

Flow cytometry is a widely used method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations and analyzing cell size and volume. It allows simultaneous multi-parameter analysis of single cells.

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What's in the guide?

  • Basics of flow cytometry instrumentation
  • Fluorophores and compensation
  • Direct vs indirect methods for antigen detection
  • Panel building, recommended controls and data analysis