Quickly set up your flow cytometry experiment with our comprehensive guide
Flow cytometry is a widely used method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations and analyzing cell size and volume. It allows simultaneous multi-parameter analysis of single cells.
What's in the guide?
Basics of flow cytometry instrumentation
Fluorophores and compensation
Direct vs indirect methods for antigen detection
Panel building, recommended controls and data analysis