Knock-out (KO) cell lines are powerful tools to study disease mechanisms, including the effect of gene disruption in signaling pathways. Download our application note to see how KO cell lines can be used to investigate signaling pathways, in this case, the TRAIL pathway.
TRAIL (TNF-related apoptosis-inducing ligand) is a protein cytokine that causes apoptosis through binding to several death receptors, including DR4 and DR5. The TRAIL-induced apoptotic pathway has recently gained substantial interest as cancer cells are significantly more sensitive to TRAIL-induced apoptosis that normal cells.
In this application note, we demonstrate that the loss of death receptor 5 (DR5) by CRISPR gene KO, blocks the downstream processing associated with TRAIL signaling, and has a greater effect that Q-DEVD-OPh, a small molecule inhibitor, at the concentrations use.
Download now
Abcam will process your personal data in strict accordance with its privacy policy which can be found here. This will include sending you updates about Abcam, our products, and resources we think would be of interest to you. You can unsubscribe and manage your marketing preferences by clicking the link at the bottom of every email we send. By filling out this form you consent to Abcam sharing your details with your local, authorized distributor for the purpose of answering your query.