Introduction to flow cytometry

Learn more about this useful technology with our introduction to flow cytometry guide.

Flow cytometry is a widely used method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations and analyzing cell size and volume. It allows simultaneous multi-parameter analysis of single cells.

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What's in the guide?

  • The flow cytometer: fluidics
  • Measuring forward and side scatter of light
  • Measuring scattered light and fluorescence
  • Antibody staining and selecting a fluorochrome conjugate