Learn more about this useful technology with our introduction to flow cytometry guide.
Flow cytometry is a widely used method for analyzing the expression of cell surface and intracellular molecules, characterizing and defining different cell types in a heterogeneous cell population, assessing the purity of isolated subpopulations and analyzing cell size and volume. It allows simultaneous multi-parameter analysis of single cells.
What's in the guide?
The flow cytometer: fluidics
Measuring forward and side scatter of light
Measuring scattered light and fluorescence
Antibody staining and selecting a fluorochrome conjugate