Western blot (WB), also called Immunoblot, uses specific antibodies to analyze one protein in a sample containing multiple proteins. Proteins are separated from each other by size using gel electrophoresis, then transferred to a membrane and detected with a specific antibody. This technique is widely used in biological research.
Review advice on sample preparation and troubleshooting tips in this webinar.
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Caitlin Buckspan received her B.S. in Biological Engineering from the University of Florida in 2007 and a M.S. in Bioengineering from the University of California, San Diego in 2010. At the University of Florida, she researched seasonal changes in termite hormone levels, where her research relied heavily on western blots.
