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Overview


An introduction to immunofluorescent array tomography, with a special user's perspective.

Review immunofluorescent array tomography- a method for studying synapses in the brain- in detail and see how it can be applied to other subcellular structures, such as mitochondria.



Topics

  • An introduction to array tomography methods
  • Choosing antibodies and troubleshooting
  • Investigating the protein composition of individual synapses
  • Further applications: Studying organelle diversity


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    Meet our presenters

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    Meet our presenters

    Kristina D. Micheva & Thomas Misgeld

    Kristina D. Micheva is a Senior Research Scientist in the Department of Molecular and Cellular Physiology at Stanford University, in California, USA. . Together with Dr. Stephen J Smith, Kristina is the co-inventor of array tomography. She has taught this imaging method at the Neurobiology Course at MBL in Woods Hole, Massachusetts for the past nine years.

    Thomas Misgeld is Professor for Neuronal Cell Biology at the Technical University Munich, Germany, and at the German Center for Neurodegenerative Diseases. He studied medicine in Munich before joining the lab of Jeff Lichtman in St. Louis and Boston as a post-doc.

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