Fusion tag finder

Consider tandem affinity purification

Not all tags are the same: they have different strengths and limitations. There are occasions where you may need a tag that can make your protein more soluble but also require a tag for purification. This is achieved by a process known as tandem affinity purification (TAP).

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Use a fluorescent tag

Fluorescent tags are non-toxic, minimally disruptive, and can be used in live cells. Although GFP is the most commonly used fluorescent tag, there is a wide variety to choose from.

Which tag is best for you?

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Use an affinity tag

Affinity tags are used to purify proteins from a cell lysate. By attaching an affinity tag to your protein of interest, it can be successfully pulled out of the solution via chemical or physical interactions with an immobilized substrate. Discover how affinity tags can boost your research in our guide.

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Use an epitope tag

Epitope tags are commonly used in antibody-based assays, such as ELISA, co-IP, and Western blot. They are generally short in length and unlikely to affect protein function. Highly specific antibodies are available against epitope tags, so they are useful tools in protein detection.

Find your epitope tag

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Consider our reagent identification program

Are you using the best reagents for your experiment? Our antibody and reagent identification program can provide you with product recommendations for your specific needs. Whether it’s searching for antibodies to multiple protein targets or other reagents for a large-scale project, we can help.

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In the middle of something? Email your result to keep it safe and discover additional useful resources.

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Struggling to identify your protein?

Do you need to view your protein in live cells?

Do you need to purify your protein or express in vivo?

Are you intending to use an antibody based assay? For example, ELISA, Western Blot, immunoprecipitation, protein localization.

Do you need to utilize multiple applications?

Is there a reliable antibody (validated for your application) available?

Do you need to utilize multiple applications?

Do you need to purify your protein?

Consider tandem affinity purification

Use a fluorescent tag

Use an affinity tag

Use an epitope tag

Consider our reagent identification program