Fluorochrome chart: a complete guide

Fluorescent dyes (also known as fluorochromes) are commonly used as detection reagents in a variety of applications such as cellular imaging and flow cytometry. Fluorochromes absorb light energy of a specific wavelength and re-emit it at a longer wavelength.

The wavelengths at which the fluorochrome absorbs and emits light constitute what is known as the fluorochrome’s excitation and emission spectra respectively (or just fluorescence spectra).

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Our chart includes

  • Aligned emission and excitation fluorescence spectra for 30 commonly-used fluorochromes

  • Easy visualization of the most popular lasers and filters across the fluorescence spectra

  • The fluorescence channel and relative brightness for each fluorochrome

  • Step by step instructions on how to use the chart effectively