Imaging quiz | Abcam

Part 1 | Part 2 | Part 3 | Part 4 | Take the quiz

Cell imaging quiz

Test your knowledge. Are you a cell imaging warrior!?

Question 1.When preparing your sample for confocal microscopy, what should you do?

a. Ensure that each protein target is separately labeled

b. Use antibodies labeled with fluorophores with spectrally separate excitation and emission spectra

c. Set the confocal filters for the fluorophores so they can be separated in the light path

d. All the above

Question 2: Which of these is showing a green autophagosome stain?

ab192890 staining LC3B in HAP1 cells

ab109110 staining EEA1 in JAR cells

ab32577 staining caveolin-1 in A431 cells

ab92547 staining vimentin in HeLa cells

Question 3: What is the main advantage of indirect compared to direct immunofluorescence?

a. Signal amplification

b. Less species cross-reactivity

c. Less flexibility

d. None of the above

Question 4: Can you identify the green endoplasmic reticulum staining?

ab14748 staining mitochondria in HeLa cells

ab92516 staining calreticulin in HAP1 cells

ab92547 staining the cytoplasm of HeLa cells

ab179513 staining beta-tubulin in HeLa cells

Question 5: Which of the following can you use as a secondary antibody when staining HeLa cells with a rabbit anti-laminin primary antibody?

a. Goat anti-rabbit IgG – H&L (Alexa Fluor^® 488)

b. Rabbit anti-goat IgG – H&L (Alexa Fluor^® 488)

c. Goat anti-mouse IgG – H&L (Alexa Fluor^® 405)

d. None of the above

Question 6: Which of these images does not show a green stain in the cell membrane?

ab92547 staining the cytoplasm of HeLa cells

ab76020 staining sodium potassium ATPase in MCF-7 cells

ab40772 staining E-cadherin in HT-29 cells

ab32577 staining caveolin-1 in A431 cells

Question 7: When designing a multicolor experiment, which of the following should you do?

a. Use any excitation and emission filter

b. Change dichroic mirrors available to ensure satisfactory fluorescent signal

c. Use dyes which emit at different wavelengths for each target protein

d. Use primary antibodies with the same isotype subtypes

Question 8: If you have weak or absent staining, which troubleshooting tip should you try?

a. Increasing the antibody concentration and incubating overnight at 4°C

b. Increasing the number of washing steps

c. Including a negative control

d. Using a different secondary antibody isotype

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