Flow cytometry quiz

Question 1. Which cell characteristic correlates with forward scatter intensity?

a. Cell size

b. Granularity

c. Cell viability

d. All the above

Question 2. What is the main purpose of using negative control in flow cytometry?

a. Set up compensation

b. Set up the gating to distinguish positive from negative population

c. Exclude dead cells from the analysis

d. All the above

Question 3. How to check if your sample exhibits autofluorescence?

a. Run an aliquot of unstained cells

b. Set up compensation

c. Use cell viability marker

d. Use isotype control

Question 4. Which step is essential to add to your flow cytometry protocol when studying intracellular proteins?

a. Staining for cell viability

b. Incubation with secondary antibodies

c. Permeabilization

d. None of the above

Question 5. What should you consider when choosing fluorophores for flow cytometry?

a. Use brighter fluorochromes for samples with low/unknown antigen expression and low cell numbers

b. Match the excitation wavelength of your fluorochromes to the lasers available and the emission wavelength of your fluorochromes to the filters

c. Avoid fluorochrome combinations with high spectral overlap

d. All the above

Question 6. How do you check for spectral overlap in multicolor flow cytometry?

a. Exclude dead cells from the analysis

b. Use isotype control

c. Run each fluorochrome separately

d. All the above

Question 7. In multicolor flow cytometry, which type of control do you need to set correct gating and distinguish a positive from a negative population?

a. Fluorescence-minus-one (FMO) control

b. Isotype control

c. Viability marker

d. All the above

Question 8. Which troubleshooting tip should you try when experiencing high fluorescence intensity in your sample?

a. Reduce antibody concentration

b. Check if the lasers are aligned correctly

c. Reduce blocking time

d. Filter cell suspension to remove the clumps

Your Score 0/8

Share result

Abcam will process your personal data in strict accordance with its privacy policy which can be found here. This will include sending you updates about Abcam, our products, and resources we think would be of interest to you. You can unsubscribe and manage your marketing preferences by clicking the link at the bottom of every email we send.