ChIP training

Part 1 | Part 2 | Part 3 | Part 4 | Take the quiz

ChIP quiz

Test your knowledge. Are you a ChIP master?!

Question 1. What is the main application of ChIP?

a. Study gene regulation

b. Detect specific proteins in the mixture

c. Amplify DNA

d. All the above

Question 2. Which step is not required for native ChIP (N-ChIP)?

a. Chromatin fragmentation

b. Immunoprecipitation

c. Cross-linking

d. DNA recovery and purification

Question 3. What is the purpose of the cross-linking step in ChIP?

a. Increase the final yield of the DNA

b. Fix chromatin-associated proteins to chromatin

c. Fragment chromatin into small pieces

d. Reduce non-specific binding

Question 4. Which of the following statements does NOT apply for the native ChIP (N-ChIP)?

a. Used for histones

b. Provides high-resolution

c. Used for non-histone proteins

d. Utilizes micrococcal nuclease for chromatin fragmentation

Question 5. What is the optimal size of chromatin fragments for ChIP?

a. 200-1000 bp

b. <100 bp

c. >1000 bp

d. >2000 bp

Question 6. Which step of ChIP-seq analysis can you use to determine the potential biological function of a transcription factor?

a. Quality checks

b. Gene ontology

c. Peak calling

d. Mapping sequencing reads

Question 7. Which aspects of ChIP protocol should you optimize when setting up a new experiment?

a. Antibody concentration

b. Chromatin preparation

c. Washing buffer

d. All the above

Question 8. Which reason explains a high background in non-specific control in a ChIP experiment?

a. Non-specific binding to the beads

b. Some beads giving high background

c. Contamination of the ChIP buffers

d. All the above

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