Western blot (WB), also called Immunoblot, uses specific antibodies to analyze one protein in a sample containing multiple proteins. Proteins are separated from each other by size using gel electrophoresis, then transferred to a membrane and detected with a specific antibody. This technique is widely used in biological research.
Listen in as our our scientific support expert, Heather Allen, reviews tips and tricks on how to plan and optimize your western blot experiments.
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Heather received a Bachelor's in Psychology from Vassar College, focusing on Physiological Psychology. She then spent 6 years working at Beth Israel Deaconess as research assistant studying chemokine signaling, relying heavily on western blot in the lab.
