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Overview


Review this powerful technique, get advice on selecting antibodies, and learn troubleshooting techniques with our Epigenetic specialist.

ChIP is a very powerful technique that allows for the analysis of protein localization on DNA regions in the genome. Selective enrichment of a chromatin fraction using antibodies allows for the isolation of DNA regions and interacting proteins.


Topics

  • What is ChIP?
  • What is chromatin?
  • Histones
  • Different types of ChIP
  • ChIP protocol: chromatin preparation, sonication, immunoprecipitation, cross-linking reversion and DNA purification, DNA analysis, needed controls


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    Meet our presenter

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    Meet our presenter

    David Grotsky

    David Grotsky received his PhD in Molecular Genetics and Genomics from Washington University in St. Louis. His PhD thesis focused on studying the regulation of DNA repair factors and how they impact genomic stability and proliferation in tumor cells.

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