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Take the quiz
Antibody basics quiz
Test your knowledge. Do you know your antibodies?
Question 1. What are the advantages of using recombinant antibodies?
a. Low batch-to-batch variability
b. Improved sensitivity
c. Animal-free production
d. All the above
Question 2. If your primary antibody is raised in mouse, which secondary antibodies can you use?
a. Goat anti-rabbit
b. Goat anti-mouse
c. Mouse anti-rabbit
d. Donkey anti-goat
Question 3. What should be your first step upon receiving your brand-new antibody?
a. Aliquot the antibody to ≥10 µL
b. Store at -20°C immediately
c. Add sodium azide to prevent microbial contamination
d. Centrifuge the antibody
Question 4. Which application should you use to study the localization of your protein of interest in tissue?
a. Flow cytometry
b. Immunohistochemistry
c. Western blot
d. ELISA
Question 5. Why knockout (KO) validation of antibodies serves as a true negative control?
a. It’s a qualitative assay
b. It detects the binding of the antibody to the target protein
c. It confirms the specificity of the antibody
d. All the above
Question 6. What is the main advantage of indirect compared to direct methods of antibody detection?
a. Signal amplification
b. Shorter time
c. Less species cross-reactivity
d. Less flexibility
Question 7. If you have weak or absent staining, which troubleshooting tip should you try?
a. Include positive control
b. Increase antibody concentration and incubate it overnight at 4°C
c. Use antigen retrieval
d. All the above
Question 8. If your staining has a high background, which troubleshooting tip should you try?
a. Decrease the blocking incubation period
b. Increase antibody concentration
c. Use the amplification technique
d. Increase the number of washing steps
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