Overview
Review this powerful technique, get advice on selecting antibodies, and learn troubleshooting techniques with our Epigenetic specialist.
ChIP is a very powerful technique that allows for the analysis of protein localization on DNA regions in the genome. Selective enrichment of a chromatin fraction using antibodies allows for the isolation of DNA regions and interacting proteins.
Topics
What is ChIP?
What is chromatin?
Histones
Different types of ChIP
ChIP protocol: chromatin preparation, sonication, immunoprecipitation, cross-linking reversion and DNA purification, DNA analysis, needed controls