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Overview


Identifying bona fide protein-protein interactions can be a difficult task due to the complexity of protein interactions.

Join our presenters as they discuss how to identify protein interactions though immunoprecipitation and overcome biochemical problems that can be associated with this technique.



Topics

  • Sample preparation
  • Positive and negative control design
  • Different binding and elution techniques
  • Upscaling of IPs
  • Validation of interactions (yeast-two hybrid/mutational analysis/mass spectrometry/western blotting)
  • Evaluation of the functional role of an interaction
  • IP hints and tips


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    Meet our presenter

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    Meet our presenter

    Seán Garry

    Seán Garry received his degree in genetics from Trinity College Dublin in 2006 and continued into research with a PhD in the laboratory of Dr. Margaret McGee at the Conway Institute, University College Dublin. For his thesis, Seán investigated how the pro-apoptotic Bcl-2 family member, Bim, is phosphorylated following prolonged mitotic arrest.

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