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Overview


Identifying bona fide protein-protein interactions can be a difficult task due to the complexity of protein interactions.

Join our presenters as they discuss how to identify protein interactions though immunoprecipitation and overcome biochemical problems that can be associated with this technique.



Topics

  • QC of sequencing reads
  • Read alignment: mapping sequencing reads to the reference genome
  • Peak calling: identify binding sites of the protein of interest
  • Data visualization
  • De novo motif discovery, gene ontology of binding sites and heatmap generation


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    Meet our presenter

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    Meet our presenter

    Xi Chen

    Xi Chen completed his undergraduate studies at the Health Science Centre in Peking University, China. He obtained his PhD in the lab of Professor Andy Sharrocks at the University of Manchester, where he investigated the DNA binding specificity of several forkhead transcription factors using both traditional biochemical assays, and state-of-the-art genomic approaches.

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